Qiagen plasmid maxi kit, 10 preps, 100 to 500ml culture volume, transfectiongrade. Plasmid dna extraction using the presto midi plasmid kit from geneaid. Qiafilter plasmid midi kit 25 from qiagen selectscience. Please consult the material safety data sheet msds for information regarding hazards. Efficient plasmid dna concentration and desalination without. Plasmid dna was purified from 25 ml and 100 ml cultures of the indicated e. Plasmids and cosmids up to 50 kb in size can be purified using hispeed plasmid kit.
Qiagen plasmid plus kits provide a novel patentpending method for extremely fast and. For qiagen plasmid midi, maxi, mega, and giga kit protocols. For the qiagen plasmid mini kit protocol microcentrifuge 1. The qiagen plasmid plus kits provide the most convenient method for largescale plasmid preparation.
Qiagen plasmid plus midi kit 25 from qiagen sample to. Qiagen plasmid plus midi kit, 100 preps, 25 to 50ml culture volume. Qiagen plasmid plus midi kit 100 from qiagen sample to. Qiagen plasmid plus maxi kit 25 input volume 50 200 ml, yield up to 1 mg. Qiagen originals pure plasmids, genuine kits learn more. Plasmid dna purification using qiagen plasmid plus midi kits 18. Yields of plasmid dna were 60100% higher with the nucleobond kits than the qiagen kits q midi qiagen plasmid midi kit, q maxi qiagen plasmid maxi kit. The pureyield system requires a vacuum pump and manifold e. Plasmid dna was purified from bacterial cultures using nucleobond xtra midi, nucleobond xtra maxi, and corresponding midiprep and maxiprep kits from qiagen following the protocols for each kit in each case, the maximum input volume specified by the corresponding kit protocol was processed. Qiagen plasmid plus kits for the fastest and most convenient purification. The purchaser must determine the suitability of the product for its particular use. The genopure plasmid midi kit prepares highly purified plasmid dna in medium quantities using a modified alkaline lysis method.
Qiagen plasmid plus purification handbook april 2012 en. Qiagen plasmid plus midi kit 100, 12945 from qiagen labsave. Spin column centrifugation and vacuum spin column centrifugation and vacuum main sample. Qiagen plasmid midi kit vs zymopure plasmid midiprep kit 25. The procedure can be performed in 20 midi and maxi, 40 mega, or 50 minutes. Qiagen plasmid plus kits provide a novel patentpending method for extremely fast and easy largescale preparation of transfectongrade plasmid dna. Qiavac 24 plus handbook 102010 7 introduction the qiavac 24 plus is designed for fast and efficient vacuum processing of up to 24 qiagen spin columns in parallel tables 1 and 2. The design and unique binding chemistry of the qiagen plasmid plus columns allow a simple bindwashelute procedure based on a novel chemistry. Qiagen plasmid plus midi kit, 100 preps, 25 to 50ml culture volume, transfectiongrade plasmid or cosmid dna purification, advanced silica technology, manual processing, 20 min. Following the purification process, a yield of more than 200.
Purify up to 20 samples 10 minutes handsontime75 minutes overall. Qiagen tips can be left unattended, since the flow of buffer will stop when the meniscus reaches the upper frit in the column. Four different cell lines cos7, hek293, hela, and huh7 were grown to 8090% confluence and transfected with 100 ng of each plasmid, in complex with 0. Genelute hp plasmid maxiprep kit user guide catalog nos. Qiagen plasmid plus midi kit 25 from qiagen selectscience. Qiagen kit handbooks and user manuals are available at. The midiprep kit protocol will typically yield 100350 g plasmid dna from 1525 ml bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. Mar, 2017 qiagen originals pure plasmids, genuine kits learn more. Midipreps for plasmid dna purificationnucleobond xtra midi. Purifying plasmid dna using the qiagen miniprep kit protocol. Thermo scientific genejet plasmid midiprep kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid dna from recombinant e. Qiagen plasmid plus midi kit, 25 preps, 25 to 50ml culture volume, transfectiongrade plasmid or cosmid dna purification, advanced silica technology, manual processing, 20 min.
I have done lots of mini preps before but this is my first midi prep. Integrated automated sample preparation and assay setup, with manual transfer to the detection unit. Qiagen plasmid midi kit vs zymopure plasmid midiprep kit 25 preps this page will help you compare kit specs and protocols across manufacturers. Qiagen plasmid plus maxi kit vs zymopure plasmid maxiprep. The geneaid midi plasmid kit uses prepacked anionexchange resin columns to purify plasmid dna from 50300 ml of cultured bacterial cells.
Qiagen plasmid maxi kit, 10 preps, 100 to 500ml culture volume, transfectiongrade plasmid or cosmid dna purification, anionexchange technology, 500g yield, 160 min. The procedure can be performed in 20 midi and maxi, 40 mega, or 50 minutes giga using a vacuum and centrifuge. Qiagen plasmid plus technology principle qiagen plasmid plus technology delivers the same performance and quality as anionexchange technology. The procedure can be performed in 20 midi and maxi. Hispeed plasmid purification handbook harvard university. Qiagen plasmid maxi kit 10, 12162 from qiagen labsave. Plasmid dna encoding constitutively expressed gfp pegfpc2 was prepared using either monarch plasmid miniprep kit or qiagen qiaprep spin miniprep kit. Qiagen plasmid plus mega kit 5 input volume 500 ml, yield up to 2. Samples and wash solutions are drawn through the column membranes by vacuum instead of centrifugation, providing greater speed and reduced handson time in. For the fastest, most convenient purification of up to 10 mg transfectiongrade plasmid dna. May 09, 2012 plasmid dna extraction using the presto midi plasmid kit from geneaid. For fast purification of up to 10 mg transfectiongrade plasmid or cosmid dna. Plasmid dna was purified from bacterial cultures using nucleobond xtra midi, nucleobond xtra maxi, and corresponding midiprep and maxiprep kits from qiagen following the protocols for each kit in.
Qiagen plasmid purification handbook qiagen plasmid midi, maxi, mega, and giga kits qiafilter plasmid midi, maxi, mega, and giga kits endofree plasmid maxi, mega, and giga kits for purification of ultrapure plasmid dna second edition august 2003. Should any product fail to perform satisfactorily due to any reason other. I have used mini preps on the exact same plasmid with no problem. Qiagen plasmid plus midi kit, 25 preps, 25 to 50ml culture volume. Qiagen plasmid mini handbook 0399 7 qiagen plasmid purification procedure plasmid sizes plasmids up to approximately 150 kb can be purified using qiagen plasmid purification protocols. Trueblue lysis buffer an optional color indicator is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. Timerun, up to 1mg yield, for the fastest, most convenient purification of up to. Trueblue lysis buffer an optional color indicator is. The kit utilizes silicabased membrane technology in the form of convenient spin column. For larger constructs qiagen plasmid kits or the largeconstruct kit are recommended plasmidcosmid copy.
Qiagen qiafilter plasmid midi kit, 25 preps, 25 to 50ml culture volume, transfectiongrade. The endotoxin content of the purified plasmid dna was determined using the kineticqcl kinetic chromogenic assay. This procedure makes these kits the fastest largescale prep available 20 minutes for maxi and midi preps. Manual centrifugation and vacuum, parallel processing, manual centrifugation and vacuum, parallel processing. Compactprep plasmid midi kit for fast purification of up to 200 g molecular biology grade plasmid dna. The purelink hipure plasmid midiprep kit is designed to isolate transfectiongrade plasmid dna from e. Manual 17 vacuum manifold 8 automated 5 biorobot automated 4 qiacube automated 1. Qiagen plasmid plus giga kit 5 input volume 2500 ml, yield.
Qiagen qiafilter plasmid midi kit, 25 preps, 25 to 50ml culture volume, transfectiongrade plasmid or cosmid dna purification, anionexchange technology, manual processing, 110 min. Qiagen plasmid purification handbook 082003 5 kit contents qiagen plasmid kits qiagen plasmid kit midi 25 midi 100 maxi 10 maxi 25 maxi 100 catalog no. Small scale purification miniprep from less than 5 ml of bacterial culture is a quick way for clone verification or dna isolation. Qiagen plasmid plus midi kit en print bookmark share pdf 57kb. Plasmid or cosmid dna purification using qiagen plasmid midi and maxi kits. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. For highthroughput manual or automated whole genome amplification from small or precious samples. Plasmid dna purified with the aurum plasmid midi kit is eluted into a small volume of aqueous buffer.
Click on the product or protocol links to be directed to the manufacturers pages. Plasmid or cosmid dna purification using qiagen hispeed. Item qiagen plasmid plus midi kit 25 company qiagen sample to insight. Plasmid dna prepared by tianprep mini plasmid kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in. Timerun, up to 1mg yield, ideal transfection of most cell lines, cloning, enzymatic. For larger constructs qiagen plasmid kits or the largeconstruct kit are recommended plasmid cosmid copy number plasmid and cosmids vary in copy number, depending on the origin of replication they contain, their size, and the size of insert. The procedure can be performed in 20 midi and maxi, 40 mega, or 50 minutes giga using a vacuum. Timerun, 100g yield, ideal for transfection, cloning, sequencing, lysate clearing without. I wonder whether the membrane column is not suitable for such a largesized plasmid, but manual isolation using alkaline lysis also gives similar result. Constructs larger than 4550 kb, however, may exhibit somewhat reduced elu tion efficiencies. The qiagen plasmid kits uses gravityflow qiagen anionexchange tips for efficient purification of plasmid dna. The aurum plasmid midi kit is optimized for the purification of up to 100 g of plasmid dna, rapidly and inexpensively, without the use of toxic reagents, highspeed centrifugations, or alcohol precipitations. Qiagen plasmid purification handbook harvard university.
1594 474 645 1259 92 21 604 398 395 791 554 630 1359 679 1186 1381 1303 1372 1177 837 1257 1309 590 754 492 69 865 964 173 434